دانلود کتاب درسی شبیه سازی، بیان و خالص سازی پروتئین های نوترکیب

The twentieth century had been a torchbearer of several seminal scientific discoveries that revolutionized the world through cogent advances in technology and biomedical sciences. It is simply intriguing to learn that identification of a small plasmid DNA in the early 1950s led to a series of scientific explorations with the consequential revelation of many important biomolecules, including restriction endonucleases and ligases. These paramount discoveries helped germinate the concept of engineering a gene simply inside a test tube. Introduction of genes that encode “proteins of interest” into plasmid DNAs, followed by their expression in any microorganism such as E. coli, and subsequent purification in vitro for research and commercial purposes, gave birth to the field of recombinant DNA technology. This steady journey since mid-twentieth century soon burgeoned exponentially over the years to bring forth radical changes in the fields of agricultural, food, and biomedical research. Furthermore, this technology has been successfully employed to develop therapeutics such as antibodies and vaccines, thus uplifting the overall quality of life. This textbook has been envisaged to provide thorough information on this important topic of biotechnology focusing on cloning, expression, and purification of heterologous target proteins primarily in the bacterial system. This book is divided into eleven chapters where each and every aspect of recombinant protein expression and purification has been chronologically and lucidly elaborated. Apart from providing a broad overview, the book annotates the recent developments in the field along with vivid protocols and methodologies to enable researchers to apply the knowledge in their own laboratory experiments. Furthermore, each chapter provides easy understanding of various concepts through user-friendly flowcharts, illustrations, troubleshooting tips, and problems. This textbook also aims at providing up-to-date information with thorough discussions on current and potential clinical applications. I am indebted to all the contributing authors for their hard work and for sharing their thoughtful insights on protein expression and purification. I also thank my other lab members and friends who spent considerable amount of time proofreading and giving invaluable inputs.