دانلود کتاب معماری کروموزوم – روش ها و پروتکل ها

Following the success of the first edition of Chromosome Architecture: Methods and Protocols as part of the Methods in Molecular Biology series, this new edition comprises updated chapters from the original authors as well as new content from new contributors in this fast-moving field. This issue, as with the original, will consist of a collection of cutting-edge laboratory protocols, techniques, and applications in use today by some of the leading international experts in the broad field of “chromosome architecture.” A key difference in emphasis, compared with the previous collections of review articles published in this area, is the emphasis on the development and application of complex techniques and protocols, which increase the physiological relevance of chromosome architecture investigation compared to the methods utilized previously; these developments are manifest both through application of far more complex bottom-up assays in vitro and through striving to maintain the native physiological context through investigation of living, functional cells. The length scale of precision of experimental protocols in this area has improved dramatically over the recent years, and many cutting-edge methods now utilize state-of-the-art single-molecule approaches, both for imaging the DNA content of chromosome and proteins that bind to DNA and for using methods that can controllably manipulate single-DNA molecules. This issue will also include more complex, physiologically representative methods to investigate chromosome architecture through the use of advanced computational methods and mathematical analysis.