دانلود کتاب ابزارهای تک مولکولی برای تجزیه و تحلیل زیستی

Single-molecule biophysics research is a highly interdisciplinarystudy that requires diverse expertise in biology, chemistry, physics,and engineering, aiming to understand biological processes at single-molecule level against ensemble averaging. Investigationsof single-molecule biophysics have enabled direct measurement ofsingle-molecule properties that were not even previously feasibleby any ensemble methods. These achievements include, but are notlimited to, direct measurement of the elastic property of an individualstrand of nucleic acids, direct manipulation of nucleic acids or proteinmolecules, optical imaging of cellular processes in a nanometerresolution, direct torque measurement of a supercoiled DNA, and several others. The fast development of the field has also stimulated the invention and evolution of a large variety of emerging single-molecule tools, which have enabled new concepts and applicationsof bioanalysis.  Though there is an urgent need to systematically summarize theseachievements, it is too much for any individual review article to achieve a full coverage with sufficient details. Written by youngexperts in the field of single-molecule research, this book aims to provide a systematic and in-depth recap of representative topicsof single-molecule bioanalysis. The book contains six chapters thatcover topics on nanopores, optical tweezers, single-molecule FRET,DNA origami sensors, magnetic tweezers, and ABEL trap. Eachchapter provides the general concept and a brief history of the methods, technical fundamentals, diversified forms of the methods,  and the representative applications of the methods. This makesthe book ideal as a textbook for a graduate-level course. In fact, thematerials in this book were indeed summarized from the lecture notes of a graduate course supervised by the book editor at NanjingUniversity since 2015.