دانلود کتاب ژنوم فضایی – روش ها و پروتکل ها

Since Emil Heitz’s description of euchromatin and heterochromatin in 1928, it has been appreciated that the spatial arrangement of genetic material within the eukaryotic nucleus is heterogeneous and highly organized. Over the decades, striking correlations with chromosome configurations and the activity of their underlying genes have been uncovered, and identifying any causal and mechanistic links is a highly active area of ongoing research. This book covers the cutting-edge techniques used to interrogate spatial genome organization and is comprised of six main parts. Studies of genome topology were revolutionized by the advent of the chromosome conformation capture (3C) technique in 2002, identifying pairs of genetic elements in close physical proximity in vivo. Part I describes the cutting-edge extensions of this pioneering method, allowing pairwise chromatin interactions to be assessed at ever-increasing coverage. Part II focuses on targeted further adaptations to the 3C-based techniques, maintaining genome-wide views of chromatin interactions (and not necessarily limited to pairwise relationships; Chapter 6) but providing high-resolution views of particular (epi)genetic features of biological interest. Moving beyond spatial associations of genetic elements in relation to each other, Part III covers techniques allowing the relationship of chromatin with non-DNA nuclear structures to be explored genome-wide. A major limitation of all of the above methods is their reliance on obtaining population-average views of large numbers of cells. To better characterize the heterogeneity and dynamics of nuclear architectures, Part IV describes single-cell technologies, and Part V covers cutting-edge approaches to visualize them by microscopy. Finally, to be able to ask functional questions about the role of spatial chromatin organization in genomic control, Part VI gives methods for acute manipulations of chromatin architecture. As for any complex biological question, no one technique can give the full answer. It is my hope that this book will be a useful resource for you to choose and perform the ones which will best help you with your research question.