دانلود کتاب ژنومیک عملکردی مخمر – روش ها و پروتکل ها

Five years after the first edition of the Yeast Functional Genomics: Methods and Protocols issue of the Methods in Molecular Biology book series came out, it seems that the topic is more relevant and livelier than ever. Not only are the basics of yeast functional genomics still extensively used by hundreds of laboratories, but new techniques appeared that scaled up the power of genome-wide approaches of yeast functioning. Third-generation sequencing devices gave a new start to yeast transcriptomics by giving access to full-length cDNAs, and even allow direct sequencing of RNAs and their chemical modifications, paving the way to yeast epitranscriptomics. Transposon saturation, RNAi and CRISPR/cas assays empowered the already high potential for global genetic screens in yeast, looking for protein-protein interactions, and essential genes or gene-drug interactions. At the same time, yeast phenomics, i.e., highthroughput yeast phenotypic profiling, which appeared at the end of the first decade of the century, has been automatized and now takes advantage of bar-coded strain collections allowing for accurate lineage tracing. Alternatives to the classic chromatin immunoprecipitation-sequencing (ChIP-seq) approach, together with single cell transcriptomics and with the development of various techniques to detect RNA polymerase II activity all along the genome, have been changing the way we envision yeast transcriptional networks. Specific ribosome profiling, tailored and more quantitative proteomics with smarter way of analyzing native protein complexes are pushing our knowledge of translational regulatory networks to the same level as that of their transcriptional counterparts.