دانلود کتاب روش و پروتکل های آسیب شناسی گیاهی

Plant pathology is an applied science that studies diseases caused by infectious microorganisms in relation to environmental and host conditions. Plant diseases are responsible of considerable economic and environmental losses in agriculture and forestry. In the last years, the impact of climate change and globalization led plant pathogens to became more harmful, posing a serious threat to food security and the environment. In this scenario, it is crucial to study the biology of plant-pathogen interaction and how this is influenced by host physiology, which is strictly related to the impact of human activities on the environment. In this changing world, the study of plant disease and the development of innovative and sustainable methods for its detection and management represent an important challenge to prevent the risk of new possible disease outbreaks in new environments, to improve plant productivity, and to protect ecosystems’ biodiversity. In recent years, the knowledge on plant diseases become increased even for the use of advanced molecular techniques that provided new insights in plant pathogen detection and plant-pathogen interaction, providing a significant support for diseases control strategies. The aim is to provide a volume that covers the latest developments in different areas of plant pathology. The contents encompass the expertise of a broad range of researchers with long expertise in plant pathology. The volume is divided into seven parts. The three chapters in Part I provide traditional methods for isolation and identification of invasive pathogens and root disease. In Part II are included three chapters on novel and rapid DNA extraction protocols from different samples (plant tissue, soil, insects). In Part III, the ten chapters are focused on molecular detection protocols (based on PCR, real-time PCR, high-resolution melting analysis, digital PCR) for identification and quantification plant pathogens, including fungal and bacterial invasive species. The three chapters of Part IV describe the application of metabarcoding in plant pathology, while in Part V, three chapters cover studies on plant-pathogen interactions. Part VI concentrates on population genomics of plant pathogens. The final part of the volume (Part VII) is devoted to biocontrol of plant pathogens. I hope this volume will be useful for the community of plant pathologists, providing innovative methods and approaches for the detection, identification, and control of plant diseases.