دانلود کتاب روشهای جدید تشخیص سریع مولکولی بیماری‌های عفونی انسانی، گیاهی، ژنتیکی و میکروبی، باکتری‌ها و ارگانیسم‌های بیماری‌زا

Different isothermal amplification methods are the signal amplification assays, the probe amplification assays, and the target amplification assays. Molecular techniques used for recognition of quinolone resistance consist of the use of various techniques such as polymerase chain reaction fragment length polymorphism, PCR, single-strand conformation polymorphism, nucleotide-sequencing analysis and multiplex allele-specific polymerase chain reaction. Main nucleic acid testing techniques are amplified nucleic acid techniques, microarrays, and non-amplified acid techniques. Nonamplified nucleic acid techniques consist of DNA labeled probes and RNA labeled probes. Molecular methods have considerable benefits for the programmatic management of drug resistance. The PCR-based methods have high sensitivity and specificity, but several molecular tests which employ non-PCR-based methods are developed for rapid detection of RNA such as isothermal nucleic acid amplification like loop mediated isothermal amplification and nucleic acid sequence-based amplification. The most notable methods which can be used to detect pathogens are biochemical assays, immunological assays and polymerase chain reaction (PCR)-based testing of bacterial nucleic acids. The reliability and sensitivity of PCR assays depend on DNA quality. The real-time PCR method can be more specific and involves complex processing steps and requires prior sequence data of the specific target gene.