دانلود کتاب روش ها و پروتکل های سلول های بنیادی پرتوان القایی (iPS)

Great strides have been made since the discovery and initial description of the developmental potential of induced pluripotent stem cells (iPS cells). From that first work, the field has exploded with new and improved methods for generation, expansion, and maintenance of iPS cells from different tissue types, characterization of their differentiation pathways along different lineages, and their potential utility in tissue repair and regeneration. In this second edition of Induced Pluripotent Stem (iPS) Cells: Methods and Protocols, we have brought together a new set of protocols to arm stem cell biologists with protocols that are currently being used in various laboratories around the world. We are hoping that people in the field will benefit from this compilation. Once again, the protocols gathered here are faithful to the mission statement of the Methods in Molecular Biology series: They are well established and described in an easy-tofollow step-by-step fashion so as to be valuable for not only experts but also novices in the stem cell field. That goal is achieved because of the generosity of the contributors who have carefully described their protocols in this volume, and we are very grateful for their efforts. Our thanks as well go to Dr. John Walker, the Editor-in-Chief of the Methods in Molecular Biology series, for giving us the opportunity to create and now update this volume, and for supporting us along the way. We are also grateful to Patrick Marton, the Executive Editor of Methods in Molecular Biology and the Springer Protocols collection, for his continuous support from idea to completion of this volume. A special thank you goes to Anna Rakovsky, Assistant Editor for Methods in Molecular Biology, for continuous support from the beginning to the end of this project. We would also like to thank David C. Casey, Senior Editor of Methods in Molecular Biology, for his outstanding editorial work during the production of this volume. Finally, we would like to thank Daniel Ignatius Jagadisan, Sarumathi Hemachandrane, Anand Venkatachalam, and the rest of the production crew for their work in putting together an outstanding volume.