دانلود کتاب سیستم تحویل ژن – فناوری تحویل نانو

Gene delivery is a transport of genes of therapeutic values into the chromosomes of the cells or tissues that can be targeted to replace faulty genes. In the last two decades, many research efforts are dedicated to gene delivery for therapeutic applications. Today, gene therapy is a promising approach in the treatment of genetic diseases, including mitochondrial related diseases like blindness, muscular dystrophy, cystic fibrosis, and some cancers. Since the first clinical trial for a treatment of combined immune deficiencies in the 1990s; over 3000 clinical trials have been approved. The delivery of genes is very complex. It entails traversing many biological barriers, and it will take years to resolve the scientific and technical issues to exploit the full potential of gene therapy. People have tried to repair genetic defects through the injection of naked plamid DNA and found it not very effective due to poor transfection efficiency. Viral vectors have shown a promising role in providing high efficiency for DNA delivery. In recent years, nanotechnology is offering the potential for the successful delivery of genes for therapeutic applications. An extensive set of nanoparticle-based delivery systems have been reported, including polymeric nanoparticles, silica-based hybrids, gold nanoparticle-based hybrids, two-dimensional nanomaterials, and lipid-based nanoparticles. Some of the interesting nano systems that are under clinical trials include DOTAP cholesterol, polyethylenimine nanoparticles, poly (ethylene glycol) polyethylenimine–cholesterol (PEG PEI Cholesterol) PEI mannose dextrose nanoparticles, and so on.