دانلود کتاب بیولومینسانس روش ها و پروتکل ها، جلد 1

Bioluminescence means a “cold light,” a unique type of chemiluminescence taking place inside a living organism. Historically, bioluminescence has been a source of fascination and curiosity for many people. Bioluminescence from living organisms is so strong as to be visible with naked eyes and intrinsically non-toxic to living organisms. Nowadays, bioluminescence is utilized as a versatile optical readout in multidisciplinary research areas, because it is advantageous in its low background intensity, high signal-tonoise ratios, wide dynamic ranges, versatility, and suitability in the imaging of animal models. Because of these distinctive virtues, bioluminescence has revolutionized molecular imaging and bioanalysis of intracellular molecular events with higher quantitative properties. In these protocol books, we highlight recent advances in molecular imaging techniques, which may be immediately useful in global biolaboratories. The chapters are categorized into nine major parts according to technological properties: (i) Establishment of Luciferins and Luciferases, (ii) Basic In Vitro Applications, (iii) Basic In Vivo Applications, (iv) Multiplex Imaging Platforms, (v) Heterogeneous Conjugates, (vi) Protein Fragment- Complementation Assays, (vii) BRET-Based Imaging, (viii) Instrumentation, and (ix) Software. We hope that this book has a role in directing and inspiring researchers to create smarter imaging techniques of the next generation, being truly quantitative, highly sensitive, and readily comprehended. All these efforts on advanced molecular imaging will engender deeper understanding of biological systems and will break new ground in the research fields of life science. I am greatly honored to complete this project with the contributors of this book. They generously accepted contributing one or multiple chapters for these books. The 66 chapters in total, to which major research groups from around the world have contributed, truly reflect the hottest research protocols regarding bioluminescence to date. I am thankful to Professor JohnWalker and the publishing team for their timely advice and support. Finally, I owe a special thank you to Young-Eun, my wife, and Yun and Hun, my children, for their endless support. I hope that this protocol book is genuinely practical and comprehensive guidance for researchers and technical staff on how to proceed with bioluminescence studies in laboratories.