دانلود کتاب پروفایلینگ ترنسکریپتوم

The genetic content of an organism is stored in the DNA of its genome and expressed through the process known as transcription. Transcriptome, the entire pool of transcripts in an organism or single cell at a certain physiological or pathological stage, is indispensable for understanding the association and regulation between DNA and protein; thus a transcriptome captures a snapshot in time of the total transcripts present in a cell. The rapid advancement of next-generation sequencing platforms, bioinformatics tools such as cloud computing, and deeper level transcriptome profiling and studies has improved our understanding of RNAbased gene mapping and regulatory networks. The transcriptomic analysis has enabled the study of how gene expression changes in different organisms and has been instrumental in the understanding of human diseases, signaling pathways regulating early embryonic development, drug safety or chemical risk assessment, and phylogenetic relationships among individuals. Furthermore, one of the world’s most serious issues is the current food crisis. The use of genetic manipulation and molecular breeding in plant science, in general, and agriculture and the food industry in particular, is an attractive and potential contribution to improving the yield, nutritional quality of plant-based foods, and medicines. The recent advances in omics such as genomics, proteomics, metabolomics, metagenomics, and transcriptomics offer the potential to speed up the process of developing crops with promising agricultural traits (e.g., heavy metal-tolerant crops, new high yielding, stress-tolerant crop varieties) compared to difficult and laborious conventional plant breeding approach. Despite the many attractive prospects offered by transcriptome sequencing on next-generation platforms, still, the development in sequencing technologies and suitable computational tools to effectively process massive amounts of sequence data continues to prompt a compilation of transcriptomics progress to date. The present book on Transcriptome Profiling: Progress and Prospects is a resource for those interested in tools and techniques used in transcriptomics, transcriptome characterization, expression analysis of transcripts, transcriptome and gene regulation, transcriptome profiling and human health, medicinal plant transcriptomics, transcriptomics and genetic engineering, and transcriptomics in agriculture. As always, I must also thank my daughter Hiba Ali and wife Sabiha Ali Ajmal for their unwavering support in the decision to spend my evenings and weekends working on this and other books.