دانلود کتاب ارزیابی ایمنی زیستی پتانسیل پروبیوتیک

There is an increasing trend in the use of probiotics as nutritional supplements and food ingredients that promote health and fight against various diseases worldwide. Researchers are pouring innovative ideas on probiotic-based research and reporting their benefits to humanity. Probiotics are sprawling their wings by their diverse applications as immune modulators, antioxidant agents, and anticarcinogenic agents, increasing the nutrient absorbing ability of host and as an ailment for various diseases. Although probiotic organisms have a wide history of safe usage, however, recent reviews and research reports have raised alarming concerns regarding the safety issues of probiotic usage. In summary, the adverse effects/negative lineages of probiotic usage include the production of harmful metabolites, excess immune stimulation, dysfunctional gut barrier, transfer of multidrugresistant genes, and risk in immunocompromised hosts which have been seriously considered at this juncture. In this context, our book entitled Biosafety Assessment of Probiotic Potential provides elaborate procedures for assessing the biosafety aspects of probiotics. This book comprises 39 protocols under two major sections dealing with in vitro biosafety assessment and in vivo biosafety assessment of probiotics. The in vitro biosafety assessment of probiotics includes various tests, including determination of biogenic amine production, D-lactic acid production, toxin production, production of various enzymes (e.g., gelatinases, glycosidases, enolase, β-glucuronidase, nitroreductase, and azoreductase), determination of toxicity, mutagenicity, presence of virulence genes, capsule formation, hemolytic activity, DNAse activity, bile salt deconjugation, antibiotic resistance and antibiotic resistance gene transfer, mucin degradation, platelet aggregation, and fibrinogen and fibronectin binding activity. The second section deals with the in vivo biosafety assessment of probiotics, which includes different tests and assays to monitor the in vivo toxicity of probiotics, assessment of infectivity in animal models, determination of reproductive and developmental toxicity in animal models, as well as evaluation of immunological parameters in animal models. These protocols are contributed by reputed senior scientists, researchers, and academicians working in these areas. We hope that the protocols presented in this book will be informative to the reader. The detailed procedures mentioned in this book are similar to a pristine stream and will be helpful to scientists, researchers, Ph.D. scholars, and postgraduate students working in this area.We express our sincere gratitude to all the authors for their excellent contributions.We are also indebted to the publishers for their efforts to publish the book in a timely manner. Criticisms and concrete suggestions are always welcome for the improvement of this book.