دانلود کتاب سنتز شیمیایی پروتئین

Protein chemical synthesis has blossomed into a highly dynamic and active research field over the past three decades. It provides new tools to generate small- to middle-sized protein samples which may be difficult for protein isolation and recombinant expression approaches, such as proteins with tailor-made natural or unnatural modification(s), labeled proteins with atomic precision, mirror image proteins, cyclic proteins, and so on. These synthetic proteins have been used to generate protein materials with new functionality and study protein posttranslational modification, structure-function relationship (e.g., phosphorylation, sulfation, glycosylation, lipidation, etc.), protein structural determination, and mirror phage display screening. Realization of protein chemical synthesis relies on the advancement of new methods and strategies to overcome the low yield and homogeneity issues of the linear solid phase peptide synthesis (SPPS) of long peptide sequence (i.e., >50 amino acids). For some short peptide sequences, SPPS is not successful, due to aggregation or self-assembling on the solid support. To this end, microwave-assisted peptide synthesis, continuous flow peptide synthesis, and new coupling conditions have been continuingly developed and advanced. To overcome the intrinsic problem of linear synthesis, convergent peptide synthesis via chemical ligation and enzymatic ligation is being widely used. In this collected work of chemical protein synthesis in Methods in Molecular Biology, seventeen chapters are presented, covering the updated development of methods, strategies, and applications of protein chemical synthesis. It will serve as a resourceful tool book for graduate students, postdoctoral fellows, and young researchers entering the field of synthetic proteins.